Physicochemical Properties and Determination of Some Bioactive Phytochemical Constituents From Linum Usitatissimum L Oil (Flaxseed) by Gas Chromatography-mass Spectrometry (GC-MS) and Atomic Absorption

Rasha Khalid Abbas1, 2
1Department of Biochemistry, Faculty of Applied and Industrial Science University of Bahri, Sudan
2Department of Chemistry, Faculty of Science and Arts in Mukhwa, University of Albaha, 65931 Saudi Arabia

DOIhttp://doi.org/10.37502/IJSMR.2021.4501

Abstract

Flaxseed (Linum usitatissimum L.) is an important source of oil, The dried seed of flaxseed 100 g, produced 38% of oil extracted by n-hexane, the physiochemical properties of flaxseed such as acid value (0.71 mg KOH/g) as oleic acid, peroxide value (2.69 meq O2/kg), saponification value (186.50 mg KOH/g oil), and iodine value (96.67 g/100 g), density (0.91 g/cm3), refractive index (1.39 and at 28°C), viscosity (CST) (68.33). Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify bioactive phytochemical constituents it contained 26 compounds identified, the major compound were 9-Octadecenoic acid (Z)-, methyl ester (Oleic acid) (43.72) act as antioxidants, anti-cancer, Hexadecanoic acid, methyl ester (Palmitic acid) (17.36) act as Anti-oxidant, decrease blood cholesterol, anti-inflammatory, 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z) fatty acids:α-Linolenic acid, an omega-3 (n-3) fatty acid γ-Linolenic acid, an omega-6 (n-6) fatty acid (15.72), Octadecanoic acid Methyl stearate (13.13) used As a food additive, soaps, cosmetics, detergents lubricants, softening and release agents,13-Docosenoic acid, methyl ester, (Z)-(1.31), cis-11-Eicosenoic acid, methyl ester (0.96) act as Antibacterial, antitumor, antifungal, cytotoxic. Atomic absorption is used to identify minerals content it contains Calcium 1180ppm, Copper 4.1ppm, Ferric 15.7ppm, Zinc 34.58ppm.

Keywords: Flaxseed (Linum usitatissimum L); physicochemical properties. Gas Chromatography-Mass Spectrometry (GC-MS): Atomic absorption.

References

  • Rubilar M, Gutiérrez C, Verdugo M, Shene C, Sineiro J. Flaxseed as a source of functional ingredients. J. Soil. Sci. Plant Nutr. 2010; 10(3):373-7.
  • , Attar-Bashi, N., & Sinclair, A. J. a-Linolenic acid and heart disease. In L. U. Thompson & S. C. Cunnane (Eds.), Flaxseed in human nutrition (2003) (2nd ed., pp. 245–259). Champaign, IL: AOCS Press.
  • Bougnoux, P., & Chajes, V. a-Linolenic acid and cancer. In L. U. Thompson & S. C. Cunnane (Eds.), Flaxseed in human nutrition (2003). (2nd ed., pp. 232–244). Champaign, IL: AOCS Press.
  • Salini T.S, Divakaran D., Shabanamol S., Sharrel R., Jisha M.S.,World J. Pharm. Res. 3 (2014) 879.
  • Ajayi G.O., Olagunju J.A., Ademuyiwa O.C., Martins O. J Med Plant Res 5 (2011) 1756.
  • Sujana N., Ramanathan S., Vimala V., Sundaram M. Inter. J. Pharm. Pharmaceutical Sci. 4 (2012) 17.
  • Panigrahi S., Sundaram Muthuraman M., Natesan R., Pemiah B. Inter. J. Pharm. Pharmaceutical Sci. 6 (2014) 93.
  • Alaa A. Gaafar1, Zeinab A. Salama1*, Mohsen S. Askar2, Dardiri M. El-Hariri3, Bakry A. Bakry3. In Vitro antioxidant and antimicrobial activities of Lignan flax seed extract (Linumusitatissimum, L.). International Journal of Pharmaceutical Sciences Review and Research. Nov – Dec 2013; nᵒ 47, 291-297
  • Kajla P, Sharma A, Sood DR. Flaxseed a potential functional food source. J. Food Sci. technol. 2015; 52(4):1857-71. Available: https://doi.org/10.1007/s13197- 014-1293-y.
  • Tohma, E. Köksal, Ö. Kılıç, Y. Alan, M.A. Yılmaz, İ. Gülçin, E. Bursal and S.H. Alwasel (2016). RPHPLC/MS/MS analysis of the phenolic compounds, antioxidant and antimicrobial activities of Salvia L. species, Antioxidants, 5, 38.
  • İ. Gülçin (2012). Antioxidant activity of food constituents-An overview, Archiv. Toxicol. 86, 345-396.
  • Topal, H. Gocer, F. Topal, P. Kalin, P. Polat Köse, İ. Gülçin, K. Cetin Çakmak, M. Küçük, L. Durmaz, A.C. Gören and S.H. Alwasel (2016). Antioxidant, antiradical and anticholinergic properties of cynarin purified from the Illyrian thistle (Onopordum Illyricum L.), J. Enzyme Inhib. Med. Chem. 31, 266-275. Material and Methods.
  • Determination of crude oil. Official methods of analysis. 17th ed. Washington: Association of Official Analytical Chemists; 2000.
  • Erwa IY, Ali AM, Khalid EA, Omer AB, Ishag OA. Proximate composition, mineral elements content and physicochemical characteristics of Adansonia digitata L seed Oil. Int J Pharma Bio Sci. 2019; 10(4):119-26.
  • ISO I. 660: 2009(E) Animal and Vegetable Fats and Oils—Determination of acid value and acidity. ISO: Geneva, Switzerland; 2009.
  • Pegg, R. (2005). Measurement of primary lipid oxidation products. In R. E. Wrolstad, T. E. Acree, & E. A. Decker (Eds.), Handbook of food analytical chemistry (pp. 515–564). New Jersey, NJ: Wiley.
  • Pearson, D. 1970. The chemical analysis of food. (6 ed). J.A. Churchill, London.
  • Wail, S.: Sue, T.T. and Yew Ai, J.1995. Palm Oil Research Institute of Malaysia. Ministry of primary Industries. Malaysia. Published by palm Oil Research Institute of Malaysia.
  • Determination of Crude Oil. Official Methods of Analysis 17th Edition, Association of Official Agric Chem. Washington; 2000.
  • Cocks, L.U.and Van Rede. 1966. Laboratory Handbook for oil and fat analysis. Academic press. Inc. Ltd. London, New York.
  • Herchi W, Al Hujaili AD, Sakouhi F, Sebei K, Trabelsi H, Kallel H, Boukhchina S. Flaxseed hull: chemical composition and antioxidant activity during development. J Oleo Sci. 2014; ess14006.
  • Rabie Y. Khattab*, Mohammad A. Zeitoun, Quality evaluation of flaxseed oil obtained by different extraction techniques, LWT – Food Science and Technology,53,(2013) 338-345.
  • Omer Adam Omer Ishag1*, Ayat A. Khalid1 , Amina Abdi1 , Ibrahim Yaagoub Erwa1 , Awadalla Babiker Omer1 and Azhari H. Nour1, Proximate Composition, Physicochemical Properties and Antioxidant Activity of Flaxseed, Annual Research & Review in Biology 34(2): 1-10, 2019.
  • G. Zou, X.L. Chen, J.N. Hu, Y.F. Wang, D.M. Gong, X.M. Zhu and Z.Y. Deng (2017). Comparisons of proximate compositions, fatty acids profile and micronutrients between fiber and oil flaxseeds (Linum usitatissimum L.). J. Food Comp. Anal. 62, 168-176.
  • Nemes SM, Orsat V, Evaluation of a microwave-assisted extraction method for lignan quantification in flaxseed cultivars and selected oil seeds. Food Anal Method, 5, 2011, 551-563.
  • Asli Can Karaca, Michael Nickerson, Nicholas H. Low, Microcapsule production employing chickpea or lentil protein isolates and maltodextrin: Physicochemical properties and oxidative protection of encapsulated flaxseed oil, Food Chemistry, 139, (2013) 448-457.
  • Kasote, D.M, Flaxseed phenolic as natural antioxidants, International Food Research Journal 20(1): 27-34 (2013).
  • Hatica Han, Hasibe Yılmaz and İlhami Gülçin, Antioxidant Activity of Flaxseed (Linum usitatissimum L.) shell and Analysis of Its Polyphenol Contents by LC-MS/MS, Rec. Nat. Prod. 12:4 (2018) 397-402.
  • Hyeon-Jeong Son and Kyung Bin Son, Antimicrobial Activity of Flaxseed Meal Extract against Escherichia coli O157:H7 and Staphylococcus aureus Inoculated on Red Mustard, J. Microbiol. Biotechnol. (2017), 27(1), 67–71.
  • Özbek, Z. Güvenalp, T. Özek, H.G. Sevindik, H. Yuca, K.Ö. Yerdelen and L.Ö. Demirezer (2017). Chemical composition, antioxidant and anticholinesterase activities of the essential oil of Origanum rotundifolium Boiss. From Turkey, Rec. Nat. Prod. 11(5), 485-490.

Works Cited

Rasha Khalid Abbas. (2021). Physicochemical Properties and Determination of Some Bioactive Phytochemical Constituents From Linum Usitatissimum L Oil (Flaxseed) by Gas Chromatography-mass Spectrometry (GC-MS) and Atomic Absorption. International Journal of Scientific and Management Research, 04(05), 1-29. doi:http://doi.org/10.37502/IJSMR.2021.4501

PDF